Origin: Streptococcus milleri S Concentration: 10000 – 30000, u.a./ml Recognition site: ATTT/AAAT TAAA/TTTA Reaction buffer: GM-buffer O + BSA, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; […]
Origin: Bacillus stearothermophilus MA Concentration: 30000-100000, u.a./ml Recognition site: GTCTC(N)1/ CAGAG(N)5/ Reaction buffer: GM-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA. ) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml […]
Origin: Bacillus schlegelii 4 Concentration: 10000 – 20000, u.a./ml Recognition site: CCNNNNN/NNGG GGNN/NNNNNCC Reaction buffer: GM-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; […]