Origin: Planococcus citreus SM Concentration: 10000 – 40000, u.a./ml Recognition site: GAATGCN/ CTTAC/GN Reaction buffer: GM-buffer O , (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 250 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; 50% glycerol. […]
Origin: Pseudomonas species L Concentration: 2000 – 5000, u.a./ml Recognition site: C/GTACG GCATG/C Reaction buffer: GM-buffer Y + BSA, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT + 100 µg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 […]
Origin: Bacillus brevis 12 Concentration: 1000 – 5000, u.a./ml Recognition site: GWGCW/C C/WCGWG Reaction buffer: GM-buffer O, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage […]