Origin: Bacillus stearothermophilus MC Concentration: 5000 – 10000, u.a./ml Recognition site: CGRY/CG GC/YRGC Reaction buffer: GM-buffer B + BSA, (10 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 1 mM DTT + 100 μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; […]
Origin: Bacillus stearothermophilus EC Concentration: 10000, u.a./ml Recognition site: C/CNNGG GGNNC/C Reaction buffer: GM-buffer Y, (33 mM Tris-acetate (pH 7.9 at 25°C); 10 mM magnesium acetate; 66 mM potassium acetate; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol. Storage […]
Origin: Bacillus stearothermophilus AC Concentration: 10000-20000, u.a./ml Recognition site: GR/CGYC CYGC/RG Reaction buffer: GM-buffer W, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 200 mM KCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% glycerol Storage temperature: -20°C […]