Origin: Bacillus stearothermophilus BA Concentration: 5000 – 20000, u.a./ml Recognition site: YAC/GTR RTG/CAY Reaction buffer: GM-buffer W + BSA, (10 mM Tris-HCl (pH 8.5 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 […]
Origin: Bacillus stearothermophilus 31 Concentration: 5000, u.a./ml Recognition site: GGTCTC(N)1/ CCAGAG(N)5/ Reaction buffer: GM-buffer O + BSA, (50 mM Tris-HCl (pH 7.6 at 25°C); 10 mM MgCl2; 100 mM NaCl; 1 mM DTT + 100μg/ml BSA.) Storage conditions: 10 mM Tris-HCl (pH 7.5); 100 mM NaCl; 0.1 mM EDTA; 7 mM 2-mercaptoethanol; 100 µg/ml BSA; […]
Origin: Bacillus pumilus Concentration: 200, u.a./ml Recognition site: CTGGAG(N)16/ GACCTC(N)14/ Reaction buffer: GM-buffer W + BSA, (10 mM Tris-HCl(pH 8.5 at 25°C); 10 mM MgCl2 ;100 mM NaCl; 1 mM DTT + 100 μg/ml BSA). Storage conditions: 10 mM Tris-HCl (pH 7.5); 50 mM KCl; 0.1 mM EDTA; 10 mM 2-mercaptoethanol; 200 µg/ml BSA; 50% […]